mouse anti ap2 α (Developmental Studies Hybridoma Bank)
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Developmental Studies Hybridoma Bank
mouse anti ap2 α
Mouse Anti Ap2 α, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+ap2+%CE%B1/anti-AP-2+alpha/bio_rxiv__2024__05__08__593219-241-58-64
Average 95 stars, based on 95 article reviews
Mouse Anti Ap2 α, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+ap2+%CE%B1/anti-AP-2+alpha/bio_rxiv__2024__05__08__593219-241-58-64
Average 95 stars, based on 95 article reviews
mouse anti ap2 α - by Bioz Stars,
2026-09
95/100 stars
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Mutagenesis:Article Title: Cadherin 4 assembles a family of color-selective retinal circuits that respond to light offset Article Snippet: Antibodies used were as follows: rabbit anti-DsRed (1:1000, Clontech Laboratories; RRID:AB_10013483); guinea pig anti-DsRed (1:500, Synaptic Systems, 390 004); chicken anti-GFP (1:1000, Abcam, Cambridge, UK; RRID:AB_300798); rat anti-Cdh4 (1:100 DSHB, RRID: AB_528110); mouse anti-Cdh13 (1:500 Santa Cruz Biotechnology, RRID:AB_10612090); goat anti-VAChT (1:1500, MilliporeSigma, Darmstadt, DE; RRID: https://identifiers.org/RRID/RRID:AB_2630394 ); guinea pig anti-RBPMS (1:100, Phosphosolutions, Aurora, CO; RRID: https://identifiers.org/RRID/RRID:AB_2492226 ); mouse anti-Ap2-α ( Article Title: The cell adhesion molecule Sdk1 shapes assembly of a retinal circuit that detects localized edges Article Snippet: Tissue was then washed with PBS and incubated in secondary antibodies for 2 hr at room temperature prior to the final wash and tissue mounting.. Antibodies and blood vessel stains Antibodies used were as follows: rabbit anti- DsRed (1:1000, Clontech Laboratories; RRID:AB_10013483); chicken anti- GFP (1:1000, Abcam, Cambridge, UK; RRID:AB_300798); mouse anti- Nr2f2 (1:1000, Abcam; RRID:AB_742211); mouse anti- Brn3c (1:250, Santa Cruz Biotechnology, Dallas, TX; RRID:AB_2167543); mouse anti- Nefh (SMI- 32, 1:1000, BioLegend, San Diego, CA; RRID:AB_2314912); rabbit anti- calbindin (1:10,000, Swant, Marly, CH; RRID:AB_2314070); goat antiosteopontin (1:1000, R&D Systems, Minneapolis, MN; RRID:AB_2194992); goat anti- VAChT (1:1000, MilliporeSigma, Darmstadt, DE; RRID:AB_2630394); rabbit anti- calretinin (1:2000, MilliporeSigma; RRID:AB_94259); guinea pig anti- vGlut3 (1:2000, MilliporeSigma; RRID:AB_2819014); guinea pig anti- RBPMS (1:100, Phosphosolutions, Aurora, CO; RRID:AB_2492226); mouse anti- Chx10 (1:300, Santa Cruz Biotechnology; RRID:AB_2216006); and mouse anti- Ap2-α ( Article Title: The cell adhesion molecule Sdk1 shapes assembly of a retinal circuit that detects localized edges Article Snippet: Antibodies used were as follows: rabbit anti-DsRed (1:1000, Clontech Laboratories; RRID :AB_10013483 ); chicken anti-GFP (1:1000, Abcam, Cambridge, UK; RRID :AB_300798 ); mouse anti-Nr2f2 (1:1000, Abcam; RRID :AB_742211 ); mouse anti-Brn3c (1:250, Santa Cruz Biotechnology, Dallas, TX; RRID :AB_2167543 ); mouse anti-Nefh (SMI-32, 1:1000, BioLegend, San Diego, CA; RRID :AB_2314912 ); rabbit anti-calbindin (1:10,000, Swant, Marly, CH; RRID :AB_2314070 ); goat anti-osteopontin (1:1000, R&D Systems, Minneapolis, MN; RRID :AB_2194992 ); goat anti-VAChT (1:1000, MilliporeSigma, Darmstadt, DE; RRID :AB_2630394 ); rabbit anti-calretinin (1:2000, MilliporeSigma; RRID: AB_94259 ); guinea pig anti-vGlut3 (1:2000, MilliporeSigma; RRID :AB_2819014 ); guinea pig anti-RBPMS (1:100, Phosphosolutions, Aurora, CO; RRID :AB_2492226 ); mouse anti-Chx10 (1:300, Santa Cruz Biotechnology; RRID: AB_2216006 ); and mouse anti-Ap2-α ( Article Title: The cell adhesion molecule Sdk1 shapes assembly of a retinal circuit that detects localized edges Article Snippet: Antibodies used were as followed: rabbit anti-DsRed (1:1000, Clontech Laboratories, RRID: AB_10013483); chicken anti-GFP (1:1000, Abcam, Cambridge, UK, RRID: AB_300798); mouse anti-Nr2f2 (1:1000, Abcam, RRID: AB_742211); mouse anti-Brn3c (1:250, Santa Cruz Biotechnology, Dallas, TX, RRID: AB_2167543); mouse anti-Nefh (SMI-32, 1:1000, Biolegend, San Diego, CA, RRID: AB_2314912); rabbit anti-Calbindin (1:10 000, Swant, Marly, CH, RRID: AB_2314070); goat anti-Osteopontin (1:1000, R&D Systems, Minneapolis, MN, RRID: AB_2194992); goat anti-VAChT (1:1000, Millipore Sigma, Darmstadt, DE, RRID: AB_2630394); rabbit anti-Calretinin (1:2000, Millipore Sigma, RRID:AB_94259); guinea pig anti-vGlut3 (1:2000, Millipore Sigma, RRID: AB_2819014); guinea pig anti-Rbpms (1:100, Phosphosolutions, Aurora, CO, RRID: AB_2492226); mouse anti-Chx10 (1:300, Santa Cruz Biotechnology, RRID:AB_2216006); mouse anti-Ap2-α ( Article Title: Notch signaling is a critical initiator of roof plate formation as revealed by the use of RNA profiling of the dorsal neural tube Article Snippet: were mounted in Prolong Diamond antifade with DAPI. .. The following primary antibodies were used: mouse anti-Tuj1, 1:750 (Sigma-Aldrich T5076); mouse anti-Isl1, 1:50 (DSHB 40.3A4); rabbit anti-cleaved Caspase-3, 1:500 (Cell Signaling Technology 9661); Article Title: Embryonic Retinal Cells and Support to Mature Retinal Neurons Article Snippet: Retinas were incubated in 300 μL primary or secondary antibodies overnight at room temperature on a rotary platform, similar to previous descriptions.. 14 Antisera and dilutions used in this study included mouse anti-2M6 at 1:80 (Paul Linser), mouse anti-AP2-α ( Sequencing:Article Title: Cadherin 4 assembles a family of color-selective retinal circuits that respond to light offset Article Snippet: Antibodies used were as follows: rabbit anti-DsRed (1:1000, Clontech Laboratories; RRID:AB_10013483); guinea pig anti-DsRed (1:500, Synaptic Systems, 390 004); chicken anti-GFP (1:1000, Abcam, Cambridge, UK; RRID:AB_300798); rat anti-Cdh4 (1:100 DSHB, RRID: AB_528110); mouse anti-Cdh13 (1:500 Santa Cruz Biotechnology, RRID:AB_10612090); goat anti-VAChT (1:1500, MilliporeSigma, Darmstadt, DE; RRID: https://identifiers.org/RRID/RRID:AB_2630394 ); guinea pig anti-RBPMS (1:100, Phosphosolutions, Aurora, CO; RRID: https://identifiers.org/RRID/RRID:AB_2492226 ); mouse anti-Ap2-α ( Article Title: The cell adhesion molecule Sdk1 shapes assembly of a retinal circuit that detects localized edges Article Snippet: Tissue was then washed with PBS and incubated in secondary antibodies for 2 hr at room temperature prior to the final wash and tissue mounting.. Antibodies and blood vessel stains Antibodies used were as follows: rabbit anti- DsRed (1:1000, Clontech Laboratories; RRID:AB_10013483); chicken anti- GFP (1:1000, Abcam, Cambridge, UK; RRID:AB_300798); mouse anti- Nr2f2 (1:1000, Abcam; RRID:AB_742211); mouse anti- Brn3c (1:250, Santa Cruz Biotechnology, Dallas, TX; RRID:AB_2167543); mouse anti- Nefh (SMI- 32, 1:1000, BioLegend, San Diego, CA; RRID:AB_2314912); rabbit anti- calbindin (1:10,000, Swant, Marly, CH; RRID:AB_2314070); goat antiosteopontin (1:1000, R&D Systems, Minneapolis, MN; RRID:AB_2194992); goat anti- VAChT (1:1000, MilliporeSigma, Darmstadt, DE; RRID:AB_2630394); rabbit anti- calretinin (1:2000, MilliporeSigma; RRID:AB_94259); guinea pig anti- vGlut3 (1:2000, MilliporeSigma; RRID:AB_2819014); guinea pig anti- RBPMS (1:100, Phosphosolutions, Aurora, CO; RRID:AB_2492226); mouse anti- Chx10 (1:300, Santa Cruz Biotechnology; RRID:AB_2216006); and mouse anti- Ap2-α ( Article Title: The cell adhesion molecule Sdk1 shapes assembly of a retinal circuit that detects localized edges Article Snippet: Antibodies used were as follows: rabbit anti-DsRed (1:1000, Clontech Laboratories; RRID :AB_10013483 ); chicken anti-GFP (1:1000, Abcam, Cambridge, UK; RRID :AB_300798 ); mouse anti-Nr2f2 (1:1000, Abcam; RRID :AB_742211 ); mouse anti-Brn3c (1:250, Santa Cruz Biotechnology, Dallas, TX; RRID :AB_2167543 ); mouse anti-Nefh (SMI-32, 1:1000, BioLegend, San Diego, CA; RRID :AB_2314912 ); rabbit anti-calbindin (1:10,000, Swant, Marly, CH; RRID :AB_2314070 ); goat anti-osteopontin (1:1000, R&D Systems, Minneapolis, MN; RRID :AB_2194992 ); goat anti-VAChT (1:1000, MilliporeSigma, Darmstadt, DE; RRID :AB_2630394 ); rabbit anti-calretinin (1:2000, MilliporeSigma; RRID: AB_94259 ); guinea pig anti-vGlut3 (1:2000, MilliporeSigma; RRID :AB_2819014 ); guinea pig anti-RBPMS (1:100, Phosphosolutions, Aurora, CO; RRID :AB_2492226 ); mouse anti-Chx10 (1:300, Santa Cruz Biotechnology; RRID: AB_2216006 ); and mouse anti-Ap2-α ( Article Title: The cell adhesion molecule Sdk1 shapes assembly of a retinal circuit that detects localized edges Article Snippet: Antibodies used were as followed: rabbit anti-DsRed (1:1000, Clontech Laboratories, RRID: AB_10013483); chicken anti-GFP (1:1000, Abcam, Cambridge, UK, RRID: AB_300798); mouse anti-Nr2f2 (1:1000, Abcam, RRID: AB_742211); mouse anti-Brn3c (1:250, Santa Cruz Biotechnology, Dallas, TX, RRID: AB_2167543); mouse anti-Nefh (SMI-32, 1:1000, Biolegend, San Diego, CA, RRID: AB_2314912); rabbit anti-Calbindin (1:10 000, Swant, Marly, CH, RRID: AB_2314070); goat anti-Osteopontin (1:1000, R&D Systems, Minneapolis, MN, RRID: AB_2194992); goat anti-VAChT (1:1000, Millipore Sigma, Darmstadt, DE, RRID: AB_2630394); rabbit anti-Calretinin (1:2000, Millipore Sigma, RRID:AB_94259); guinea pig anti-vGlut3 (1:2000, Millipore Sigma, RRID: AB_2819014); guinea pig anti-Rbpms (1:100, Phosphosolutions, Aurora, CO, RRID: AB_2492226); mouse anti-Chx10 (1:300, Santa Cruz Biotechnology, RRID:AB_2216006); mouse anti-Ap2-α ( Article Title: Notch signaling is a critical initiator of roof plate formation as revealed by the use of RNA profiling of the dorsal neural tube Article Snippet: were mounted in Prolong Diamond antifade with DAPI. .. The following primary antibodies were used: mouse anti-Tuj1, 1:750 (Sigma-Aldrich T5076); mouse anti-Isl1, 1:50 (DSHB 40.3A4); rabbit anti-cleaved Caspase-3, 1:500 (Cell Signaling Technology 9661); Article Title: Embryonic Retinal Cells and Support to Mature Retinal Neurons Article Snippet: Retinas were incubated in 300 μL primary or secondary antibodies overnight at room temperature on a rotary platform, similar to previous descriptions.. 14 Antisera and dilutions used in this study included mouse anti-2M6 at 1:80 (Paul Linser), mouse anti-AP2-α ( SYBR Green Assay:Article Title: Cadherin 4 assembles a family of color-selective retinal circuits that respond to light offset Article Snippet: Antibodies used were as follows: rabbit anti-DsRed (1:1000, Clontech Laboratories; RRID:AB_10013483); guinea pig anti-DsRed (1:500, Synaptic Systems, 390 004); chicken anti-GFP (1:1000, Abcam, Cambridge, UK; RRID:AB_300798); rat anti-Cdh4 (1:100 DSHB, RRID: AB_528110); mouse anti-Cdh13 (1:500 Santa Cruz Biotechnology, RRID:AB_10612090); goat anti-VAChT (1:1500, MilliporeSigma, Darmstadt, DE; RRID: https://identifiers.org/RRID/RRID:AB_2630394 ); guinea pig anti-RBPMS (1:100, Phosphosolutions, Aurora, CO; RRID: https://identifiers.org/RRID/RRID:AB_2492226 ); mouse anti-Ap2-α ( Article Title: The cell adhesion molecule Sdk1 shapes assembly of a retinal circuit that detects localized edges Article Snippet: Tissue was then washed with PBS and incubated in secondary antibodies for 2 hr at room temperature prior to the final wash and tissue mounting.. Antibodies and blood vessel stains Antibodies used were as follows: rabbit anti- DsRed (1:1000, Clontech Laboratories; RRID:AB_10013483); chicken anti- GFP (1:1000, Abcam, Cambridge, UK; RRID:AB_300798); mouse anti- Nr2f2 (1:1000, Abcam; RRID:AB_742211); mouse anti- Brn3c (1:250, Santa Cruz Biotechnology, Dallas, TX; RRID:AB_2167543); mouse anti- Nefh (SMI- 32, 1:1000, BioLegend, San Diego, CA; RRID:AB_2314912); rabbit anti- calbindin (1:10,000, Swant, Marly, CH; RRID:AB_2314070); goat antiosteopontin (1:1000, R&D Systems, Minneapolis, MN; RRID:AB_2194992); goat anti- VAChT (1:1000, MilliporeSigma, Darmstadt, DE; RRID:AB_2630394); rabbit anti- calretinin (1:2000, MilliporeSigma; RRID:AB_94259); guinea pig anti- vGlut3 (1:2000, MilliporeSigma; RRID:AB_2819014); guinea pig anti- RBPMS (1:100, Phosphosolutions, Aurora, CO; RRID:AB_2492226); mouse anti- Chx10 (1:300, Santa Cruz Biotechnology; RRID:AB_2216006); and mouse anti- Ap2-α ( Article Title: The cell adhesion molecule Sdk1 shapes assembly of a retinal circuit that detects localized edges Article Snippet: Antibodies used were as follows: rabbit anti-DsRed (1:1000, Clontech Laboratories; RRID :AB_10013483 ); chicken anti-GFP (1:1000, Abcam, Cambridge, UK; RRID :AB_300798 ); mouse anti-Nr2f2 (1:1000, Abcam; RRID :AB_742211 ); mouse anti-Brn3c (1:250, Santa Cruz Biotechnology, Dallas, TX; RRID :AB_2167543 ); mouse anti-Nefh (SMI-32, 1:1000, BioLegend, San Diego, CA; RRID :AB_2314912 ); rabbit anti-calbindin (1:10,000, Swant, Marly, CH; RRID :AB_2314070 ); goat anti-osteopontin (1:1000, R&D Systems, Minneapolis, MN; RRID :AB_2194992 ); goat anti-VAChT (1:1000, MilliporeSigma, Darmstadt, DE; RRID :AB_2630394 ); rabbit anti-calretinin (1:2000, MilliporeSigma; RRID: AB_94259 ); guinea pig anti-vGlut3 (1:2000, MilliporeSigma; RRID :AB_2819014 ); guinea pig anti-RBPMS (1:100, Phosphosolutions, Aurora, CO; RRID :AB_2492226 ); mouse anti-Chx10 (1:300, Santa Cruz Biotechnology; RRID: AB_2216006 ); and mouse anti-Ap2-α ( Article Title: The cell adhesion molecule Sdk1 shapes assembly of a retinal circuit that detects localized edges Article Snippet: Antibodies used were as followed: rabbit anti-DsRed (1:1000, Clontech Laboratories, RRID: AB_10013483); chicken anti-GFP (1:1000, Abcam, Cambridge, UK, RRID: AB_300798); mouse anti-Nr2f2 (1:1000, Abcam, RRID: AB_742211); mouse anti-Brn3c (1:250, Santa Cruz Biotechnology, Dallas, TX, RRID: AB_2167543); mouse anti-Nefh (SMI-32, 1:1000, Biolegend, San Diego, CA, RRID: AB_2314912); rabbit anti-Calbindin (1:10 000, Swant, Marly, CH, RRID: AB_2314070); goat anti-Osteopontin (1:1000, R&D Systems, Minneapolis, MN, RRID: AB_2194992); goat anti-VAChT (1:1000, Millipore Sigma, Darmstadt, DE, RRID: AB_2630394); rabbit anti-Calretinin (1:2000, Millipore Sigma, RRID:AB_94259); guinea pig anti-vGlut3 (1:2000, Millipore Sigma, RRID: AB_2819014); guinea pig anti-Rbpms (1:100, Phosphosolutions, Aurora, CO, RRID: AB_2492226); mouse anti-Chx10 (1:300, Santa Cruz Biotechnology, RRID:AB_2216006); mouse anti-Ap2-α ( Article Title: Notch signaling is a critical initiator of roof plate formation as revealed by the use of RNA profiling of the dorsal neural tube Article Snippet: were mounted in Prolong Diamond antifade with DAPI. .. The following primary antibodies were used: mouse anti-Tuj1, 1:750 (Sigma-Aldrich T5076); mouse anti-Isl1, 1:50 (DSHB 40.3A4); rabbit anti-cleaved Caspase-3, 1:500 (Cell Signaling Technology 9661); Article Title: Embryonic Retinal Cells and Support to Mature Retinal Neurons Article Snippet: Retinas were incubated in 300 μL primary or secondary antibodies overnight at room temperature on a rotary platform, similar to previous descriptions.. 14 Antisera and dilutions used in this study included mouse anti-2M6 at 1:80 (Paul Linser), mouse anti-AP2-α ( Recombinant:Article Title: Cadherin 4 assembles a family of color-selective retinal circuits that respond to light offset Article Snippet: Antibodies used were as follows: rabbit anti-DsRed (1:1000, Clontech Laboratories; RRID:AB_10013483); guinea pig anti-DsRed (1:500, Synaptic Systems, 390 004); chicken anti-GFP (1:1000, Abcam, Cambridge, UK; RRID:AB_300798); rat anti-Cdh4 (1:100 DSHB, RRID: AB_528110); mouse anti-Cdh13 (1:500 Santa Cruz Biotechnology, RRID:AB_10612090); goat anti-VAChT (1:1500, MilliporeSigma, Darmstadt, DE; RRID: https://identifiers.org/RRID/RRID:AB_2630394 ); guinea pig anti-RBPMS (1:100, Phosphosolutions, Aurora, CO; RRID: https://identifiers.org/RRID/RRID:AB_2492226 ); mouse anti-Ap2-α ( Article Title: The cell adhesion molecule Sdk1 shapes assembly of a retinal circuit that detects localized edges Article Snippet: Tissue was then washed with PBS and incubated in secondary antibodies for 2 hr at room temperature prior to the final wash and tissue mounting.. Antibodies and blood vessel stains Antibodies used were as follows: rabbit anti- DsRed (1:1000, Clontech Laboratories; RRID:AB_10013483); chicken anti- GFP (1:1000, Abcam, Cambridge, UK; RRID:AB_300798); mouse anti- Nr2f2 (1:1000, Abcam; RRID:AB_742211); mouse anti- Brn3c (1:250, Santa Cruz Biotechnology, Dallas, TX; RRID:AB_2167543); mouse anti- Nefh (SMI- 32, 1:1000, BioLegend, San Diego, CA; RRID:AB_2314912); rabbit anti- calbindin (1:10,000, Swant, Marly, CH; RRID:AB_2314070); goat antiosteopontin (1:1000, R&D Systems, Minneapolis, MN; RRID:AB_2194992); goat anti- VAChT (1:1000, MilliporeSigma, Darmstadt, DE; RRID:AB_2630394); rabbit anti- calretinin (1:2000, MilliporeSigma; RRID:AB_94259); guinea pig anti- vGlut3 (1:2000, MilliporeSigma; RRID:AB_2819014); guinea pig anti- RBPMS (1:100, Phosphosolutions, Aurora, CO; RRID:AB_2492226); mouse anti- Chx10 (1:300, Santa Cruz Biotechnology; RRID:AB_2216006); and mouse anti- Ap2-α ( Article Title: The cell adhesion molecule Sdk1 shapes assembly of a retinal circuit that detects localized edges Article Snippet: Antibodies used were as follows: rabbit anti-DsRed (1:1000, Clontech Laboratories; RRID :AB_10013483 ); chicken anti-GFP (1:1000, Abcam, Cambridge, UK; RRID :AB_300798 ); mouse anti-Nr2f2 (1:1000, Abcam; RRID :AB_742211 ); mouse anti-Brn3c (1:250, Santa Cruz Biotechnology, Dallas, TX; RRID :AB_2167543 ); mouse anti-Nefh (SMI-32, 1:1000, BioLegend, San Diego, CA; RRID :AB_2314912 ); rabbit anti-calbindin (1:10,000, Swant, Marly, CH; RRID :AB_2314070 ); goat anti-osteopontin (1:1000, R&D Systems, Minneapolis, MN; RRID :AB_2194992 ); goat anti-VAChT (1:1000, MilliporeSigma, Darmstadt, DE; RRID :AB_2630394 ); rabbit anti-calretinin (1:2000, MilliporeSigma; RRID: AB_94259 ); guinea pig anti-vGlut3 (1:2000, MilliporeSigma; RRID :AB_2819014 ); guinea pig anti-RBPMS (1:100, Phosphosolutions, Aurora, CO; RRID :AB_2492226 ); mouse anti-Chx10 (1:300, Santa Cruz Biotechnology; RRID: AB_2216006 ); and mouse anti-Ap2-α ( Article Title: The cell adhesion molecule Sdk1 shapes assembly of a retinal circuit that detects localized edges Article Snippet: Antibodies used were as followed: rabbit anti-DsRed (1:1000, Clontech Laboratories, RRID: AB_10013483); chicken anti-GFP (1:1000, Abcam, Cambridge, UK, RRID: AB_300798); mouse anti-Nr2f2 (1:1000, Abcam, RRID: AB_742211); mouse anti-Brn3c (1:250, Santa Cruz Biotechnology, Dallas, TX, RRID: AB_2167543); mouse anti-Nefh (SMI-32, 1:1000, Biolegend, San Diego, CA, RRID: AB_2314912); rabbit anti-Calbindin (1:10 000, Swant, Marly, CH, RRID: AB_2314070); goat anti-Osteopontin (1:1000, R&D Systems, Minneapolis, MN, RRID: AB_2194992); goat anti-VAChT (1:1000, Millipore Sigma, Darmstadt, DE, RRID: AB_2630394); rabbit anti-Calretinin (1:2000, Millipore Sigma, RRID:AB_94259); guinea pig anti-vGlut3 (1:2000, Millipore Sigma, RRID: AB_2819014); guinea pig anti-Rbpms (1:100, Phosphosolutions, Aurora, CO, RRID: AB_2492226); mouse anti-Chx10 (1:300, Santa Cruz Biotechnology, RRID:AB_2216006); mouse anti-Ap2-α ( Article Title: Notch signaling is a critical initiator of roof plate formation as revealed by the use of RNA profiling of the dorsal neural tube Article Snippet: were mounted in Prolong Diamond antifade with DAPI. .. The following primary antibodies were used: mouse anti-Tuj1, 1:750 (Sigma-Aldrich T5076); mouse anti-Isl1, 1:50 (DSHB 40.3A4); rabbit anti-cleaved Caspase-3, 1:500 (Cell Signaling Technology 9661); Article Title: Embryonic Retinal Cells and Support to Mature Retinal Neurons Article Snippet: Retinas were incubated in 300 μL primary or secondary antibodies overnight at room temperature on a rotary platform, similar to previous descriptions.. 14 Antisera and dilutions used in this study included mouse anti-2M6 at 1:80 (Paul Linser), mouse anti-AP2-α ( Plasmid Preparation:Article Title: Cadherin 4 assembles a family of color-selective retinal circuits that respond to light offset Article Snippet: Antibodies used were as follows: rabbit anti-DsRed (1:1000, Clontech Laboratories; RRID:AB_10013483); guinea pig anti-DsRed (1:500, Synaptic Systems, 390 004); chicken anti-GFP (1:1000, Abcam, Cambridge, UK; RRID:AB_300798); rat anti-Cdh4 (1:100 DSHB, RRID: AB_528110); mouse anti-Cdh13 (1:500 Santa Cruz Biotechnology, RRID:AB_10612090); goat anti-VAChT (1:1500, MilliporeSigma, Darmstadt, DE; RRID: https://identifiers.org/RRID/RRID:AB_2630394 ); guinea pig anti-RBPMS (1:100, Phosphosolutions, Aurora, CO; RRID: https://identifiers.org/RRID/RRID:AB_2492226 ); mouse anti-Ap2-α ( Article Title: The cell adhesion molecule Sdk1 shapes assembly of a retinal circuit that detects localized edges Article Snippet: Tissue was then washed with PBS and incubated in secondary antibodies for 2 hr at room temperature prior to the final wash and tissue mounting.. Antibodies and blood vessel stains Antibodies used were as follows: rabbit anti- DsRed (1:1000, Clontech Laboratories; RRID:AB_10013483); chicken anti- GFP (1:1000, Abcam, Cambridge, UK; RRID:AB_300798); mouse anti- Nr2f2 (1:1000, Abcam; RRID:AB_742211); mouse anti- Brn3c (1:250, Santa Cruz Biotechnology, Dallas, TX; RRID:AB_2167543); mouse anti- Nefh (SMI- 32, 1:1000, BioLegend, San Diego, CA; RRID:AB_2314912); rabbit anti- calbindin (1:10,000, Swant, Marly, CH; RRID:AB_2314070); goat antiosteopontin (1:1000, R&D Systems, Minneapolis, MN; RRID:AB_2194992); goat anti- VAChT (1:1000, MilliporeSigma, Darmstadt, DE; RRID:AB_2630394); rabbit anti- calretinin (1:2000, MilliporeSigma; RRID:AB_94259); guinea pig anti- vGlut3 (1:2000, MilliporeSigma; RRID:AB_2819014); guinea pig anti- RBPMS (1:100, Phosphosolutions, Aurora, CO; RRID:AB_2492226); mouse anti- Chx10 (1:300, Santa Cruz Biotechnology; RRID:AB_2216006); and mouse anti- Ap2-α ( Article Title: The cell adhesion molecule Sdk1 shapes assembly of a retinal circuit that detects localized edges Article Snippet: Antibodies used were as follows: rabbit anti-DsRed (1:1000, Clontech Laboratories; RRID :AB_10013483 ); chicken anti-GFP (1:1000, Abcam, Cambridge, UK; RRID :AB_300798 ); mouse anti-Nr2f2 (1:1000, Abcam; RRID :AB_742211 ); mouse anti-Brn3c (1:250, Santa Cruz Biotechnology, Dallas, TX; RRID :AB_2167543 ); mouse anti-Nefh (SMI-32, 1:1000, BioLegend, San Diego, CA; RRID :AB_2314912 ); rabbit anti-calbindin (1:10,000, Swant, Marly, CH; RRID :AB_2314070 ); goat anti-osteopontin (1:1000, R&D Systems, Minneapolis, MN; RRID :AB_2194992 ); goat anti-VAChT (1:1000, MilliporeSigma, Darmstadt, DE; RRID :AB_2630394 ); rabbit anti-calretinin (1:2000, MilliporeSigma; RRID: AB_94259 ); guinea pig anti-vGlut3 (1:2000, MilliporeSigma; RRID :AB_2819014 ); guinea pig anti-RBPMS (1:100, Phosphosolutions, Aurora, CO; RRID :AB_2492226 ); mouse anti-Chx10 (1:300, Santa Cruz Biotechnology; RRID: AB_2216006 ); and mouse anti-Ap2-α ( Article Title: The cell adhesion molecule Sdk1 shapes assembly of a retinal circuit that detects localized edges Article Snippet: Antibodies used were as followed: rabbit anti-DsRed (1:1000, Clontech Laboratories, RRID: AB_10013483); chicken anti-GFP (1:1000, Abcam, Cambridge, UK, RRID: AB_300798); mouse anti-Nr2f2 (1:1000, Abcam, RRID: AB_742211); mouse anti-Brn3c (1:250, Santa Cruz Biotechnology, Dallas, TX, RRID: AB_2167543); mouse anti-Nefh (SMI-32, 1:1000, Biolegend, San Diego, CA, RRID: AB_2314912); rabbit anti-Calbindin (1:10 000, Swant, Marly, CH, RRID: AB_2314070); goat anti-Osteopontin (1:1000, R&D Systems, Minneapolis, MN, RRID: AB_2194992); goat anti-VAChT (1:1000, Millipore Sigma, Darmstadt, DE, RRID: AB_2630394); rabbit anti-Calretinin (1:2000, Millipore Sigma, RRID:AB_94259); guinea pig anti-vGlut3 (1:2000, Millipore Sigma, RRID: AB_2819014); guinea pig anti-Rbpms (1:100, Phosphosolutions, Aurora, CO, RRID: AB_2492226); mouse anti-Chx10 (1:300, Santa Cruz Biotechnology, RRID:AB_2216006); mouse anti-Ap2-α ( Article Title: Notch signaling is a critical initiator of roof plate formation as revealed by the use of RNA profiling of the dorsal neural tube Article Snippet: were mounted in Prolong Diamond antifade with DAPI. .. The following primary antibodies were used: mouse anti-Tuj1, 1:750 (Sigma-Aldrich T5076); mouse anti-Isl1, 1:50 (DSHB 40.3A4); rabbit anti-cleaved Caspase-3, 1:500 (Cell Signaling Technology 9661); Article Title: Embryonic Retinal Cells and Support to Mature Retinal Neurons Article Snippet: Retinas were incubated in 300 μL primary or secondary antibodies overnight at room temperature on a rotary platform, similar to previous descriptions.. 14 Antisera and dilutions used in this study included mouse anti-2M6 at 1:80 (Paul Linser), mouse anti-AP2-α ( Software:Article Title: Cadherin 4 assembles a family of color-selective retinal circuits that respond to light offset Article Snippet: Antibodies used were as follows: rabbit anti-DsRed (1:1000, Clontech Laboratories; RRID:AB_10013483); guinea pig anti-DsRed (1:500, Synaptic Systems, 390 004); chicken anti-GFP (1:1000, Abcam, Cambridge, UK; RRID:AB_300798); rat anti-Cdh4 (1:100 DSHB, RRID: AB_528110); mouse anti-Cdh13 (1:500 Santa Cruz Biotechnology, RRID:AB_10612090); goat anti-VAChT (1:1500, MilliporeSigma, Darmstadt, DE; RRID: https://identifiers.org/RRID/RRID:AB_2630394 ); guinea pig anti-RBPMS (1:100, Phosphosolutions, Aurora, CO; RRID: https://identifiers.org/RRID/RRID:AB_2492226 ); mouse anti-Ap2-α ( Article Title: The cell adhesion molecule Sdk1 shapes assembly of a retinal circuit that detects localized edges Article Snippet: Tissue was then washed with PBS and incubated in secondary antibodies for 2 hr at room temperature prior to the final wash and tissue mounting.. Antibodies and blood vessel stains Antibodies used were as follows: rabbit anti- DsRed (1:1000, Clontech Laboratories; RRID:AB_10013483); chicken anti- GFP (1:1000, Abcam, Cambridge, UK; RRID:AB_300798); mouse anti- Nr2f2 (1:1000, Abcam; RRID:AB_742211); mouse anti- Brn3c (1:250, Santa Cruz Biotechnology, Dallas, TX; RRID:AB_2167543); mouse anti- Nefh (SMI- 32, 1:1000, BioLegend, San Diego, CA; RRID:AB_2314912); rabbit anti- calbindin (1:10,000, Swant, Marly, CH; RRID:AB_2314070); goat antiosteopontin (1:1000, R&D Systems, Minneapolis, MN; RRID:AB_2194992); goat anti- VAChT (1:1000, MilliporeSigma, Darmstadt, DE; RRID:AB_2630394); rabbit anti- calretinin (1:2000, MilliporeSigma; RRID:AB_94259); guinea pig anti- vGlut3 (1:2000, MilliporeSigma; RRID:AB_2819014); guinea pig anti- RBPMS (1:100, Phosphosolutions, Aurora, CO; RRID:AB_2492226); mouse anti- Chx10 (1:300, Santa Cruz Biotechnology; RRID:AB_2216006); and mouse anti- Ap2-α ( Article Title: The cell adhesion molecule Sdk1 shapes assembly of a retinal circuit that detects localized edges Article Snippet: Antibodies used were as follows: rabbit anti-DsRed (1:1000, Clontech Laboratories; RRID :AB_10013483 ); chicken anti-GFP (1:1000, Abcam, Cambridge, UK; RRID :AB_300798 ); mouse anti-Nr2f2 (1:1000, Abcam; RRID :AB_742211 ); mouse anti-Brn3c (1:250, Santa Cruz Biotechnology, Dallas, TX; RRID :AB_2167543 ); mouse anti-Nefh (SMI-32, 1:1000, BioLegend, San Diego, CA; RRID :AB_2314912 ); rabbit anti-calbindin (1:10,000, Swant, Marly, CH; RRID :AB_2314070 ); goat anti-osteopontin (1:1000, R&D Systems, Minneapolis, MN; RRID :AB_2194992 ); goat anti-VAChT (1:1000, MilliporeSigma, Darmstadt, DE; RRID :AB_2630394 ); rabbit anti-calretinin (1:2000, MilliporeSigma; RRID: AB_94259 ); guinea pig anti-vGlut3 (1:2000, MilliporeSigma; RRID :AB_2819014 ); guinea pig anti-RBPMS (1:100, Phosphosolutions, Aurora, CO; RRID :AB_2492226 ); mouse anti-Chx10 (1:300, Santa Cruz Biotechnology; RRID: AB_2216006 ); and mouse anti-Ap2-α ( Article Title: The cell adhesion molecule Sdk1 shapes assembly of a retinal circuit that detects localized edges Article Snippet: Antibodies used were as followed: rabbit anti-DsRed (1:1000, Clontech Laboratories, RRID: AB_10013483); chicken anti-GFP (1:1000, Abcam, Cambridge, UK, RRID: AB_300798); mouse anti-Nr2f2 (1:1000, Abcam, RRID: AB_742211); mouse anti-Brn3c (1:250, Santa Cruz Biotechnology, Dallas, TX, RRID: AB_2167543); mouse anti-Nefh (SMI-32, 1:1000, Biolegend, San Diego, CA, RRID: AB_2314912); rabbit anti-Calbindin (1:10 000, Swant, Marly, CH, RRID: AB_2314070); goat anti-Osteopontin (1:1000, R&D Systems, Minneapolis, MN, RRID: AB_2194992); goat anti-VAChT (1:1000, Millipore Sigma, Darmstadt, DE, RRID: AB_2630394); rabbit anti-Calretinin (1:2000, Millipore Sigma, RRID:AB_94259); guinea pig anti-vGlut3 (1:2000, Millipore Sigma, RRID: AB_2819014); guinea pig anti-Rbpms (1:100, Phosphosolutions, Aurora, CO, RRID: AB_2492226); mouse anti-Chx10 (1:300, Santa Cruz Biotechnology, RRID:AB_2216006); mouse anti-Ap2-α ( Article Title: Notch signaling is a critical initiator of roof plate formation as revealed by the use of RNA profiling of the dorsal neural tube Article Snippet: were mounted in Prolong Diamond antifade with DAPI. .. The following primary antibodies were used: mouse anti-Tuj1, 1:750 (Sigma-Aldrich T5076); mouse anti-Isl1, 1:50 (DSHB 40.3A4); rabbit anti-cleaved Caspase-3, 1:500 (Cell Signaling Technology 9661); Article Title: Embryonic Retinal Cells and Support to Mature Retinal Neurons Article Snippet: Retinas were incubated in 300 μL primary or secondary antibodies overnight at room temperature on a rotary platform, similar to previous descriptions.. 14 Antisera and dilutions used in this study included mouse anti-2M6 at 1:80 (Paul Linser), mouse anti-AP2-α ( |